Human RAF1-RBD is supplied as a recombinant biotinylated RAS binding domain in a 0.1 mg format for studying interactions between RAS family proteins and the RAF effector interface. Applications include measurement of GTP/GDP bound ratio of Ras in vitro, quantitation of GTP-bound Ras from lysates, RAS/RAF binding studies. Technical specifications are included below.- Quantity - 0.1 mg- Gene - RAF1- Mutation / Variant - Ras-binding domain- Organism - Human- Tag - N-terminal 6XHis and AviTag- Expression System - E. coli- Purity - greater than 90% by Coomassie staining- UniProt ID - P04049- Amino Acid Range - 50-140- Buffer - 20 mM HEPES, pH 7.5, 150 mM NaCl, 5 mM 2-mercaptoethanol, 1 mM MgCl2, 10% glycerol- Storage - -80 C- Biotinylated - Yes, N-terminusThis product is for research use only.